BIOTECNOLOGIE MICROBICHE E AGROALIMENTARI
Module BIOPROCESSI PER L'INDUSTRIA AGRO-ALIMENTARE

Academic Year 2024/2025 - Teacher: LUCIA PARAFATI

Expected Learning Outcomes

The course aims to provide the necessary knowledge for the use of microorganisms in the production of molecules of industrial interest and unitary processes and operations of the food industry.

In relation to the Dublin Descriptors 1 (Knowledge and understanding) and 2 (Ability to apply knowledge and understanding), the course aims to provide students with the necessary knowledge in order to know which products can be obtained from "bioprocesses" and understand them. the application potential. In relation to the Dublin Descriptors 3 (Judgment autonomy), 4 (Communication skills) and 5 (Learning skills), the course aims to make students acquire the ability to analyze and understand fermentation processes based on activity of microorganisms. Deepening what has been learned in the course, the student will be able to use the basic knowledge as a starting point for subsequent studies on bioprocesses that allow to obtain a wide range of products of industrial interest.

Upon completion of the course, students must have acquired independent and critical investigation skills as well as the ability to discuss and present the results of these studies.

Course Structure

The course consists of 63 hours (6 CFU) divided into21 hours of participatory and interactive lessons, with exposure through ppt. The remaining 42 hours are for exercises, during which the following are foreseen: laboratory exercises, possible technical visits to companies in the agri-food sector that use biotechnologies;

If the teaching is given in a mixed or remote mode, the necessary changes with respect to what was previously stated may be introduced, in order to comply with the program envisaged and reported in the syllabus.

Information for students with disabilities and/or DSA 

To guarantee equal opportunities and in compliance with the laws in force, interested students can request a personal interview in order to plan any compensatory and/or dispensatory measures, based on the educational objectives and specific needs.

Required Prerequisites

Knowledge of the basic contents concerning general microbiology is strongly recommended

Attendance of Lessons

Strongly recommended but not mandatory

Detailed Course Content

1. Definition of "Bioprocesses" and fields of application

2. Classification of products obtainable by applying a bioprocess

3. The fermenter and the substrates that can be used in an industrial bioprocess

4. Classification of fermentation processes

5. Case studies concerning the obtaining of products using a bioprocess

Textbook Information

Text 1. M. Manzoni. “Microbiologia Industriale”. Casa Editrice Ambrosiana.

Text 2. Michele M. Bianchi. ‘’Chimica e Biotecnologia delle fermentazioni’’-. Edizioni Nuova Cultura

Course Planning

 SubjectsText References
1Biotechnologies and bioprocesses: definition and fields of application. Microorganisms used in biotechnological and industrial applicationsText 1 - Text 2 - Material provided by the teacher
2Products obtainable from microbial biotechnologies: microbial biomass, biomass extraction products, secondary metabolites, etc.Text 1 - Material provided by the teacher
3The cultivation substrates: composition of the cultivation soils and management of raw materials. The fermenter.Text 1 - Material provided by the teacher
4Growth of microorganisms: growth curve, factors affecting growth, evaluation of microbial developmentText 1 -  Material provided by the teacher
5Classification of fermentations, batch, fed-batch and continuous culturesText 1 - Text 2 - Material provided by the teacher
6Metabolic accumulations of industrial interest Procedures for obtaining microbial metabolitesText 1 -  Material provided by the teacher
7Isolation of fermentation products: recovery and purificationText 1 -  Material provided by the teacher
8Microbial cell immobilization techniques: active and passiveText 1 -  Material provided by the teacher
9Enzymatic technologies: classification of enzymes, enzymes in solution and immobilized, use of enzymes in industryText 1 - Text 2 - Material provided by the teacher
10Use of biotechnologies for food production and / or processing (complex products) and example of complex products such as yogurt, kefir and beerText 1 -  Material provided by the teacher
11Case studies of industrial processes and products for obtaining: microbial biomass, organic acids, ethanol, etc.Text 1 -  Material provided by the teacher
12Laboratory exercises

Learning Assessment

Learning Assessment Procedures

Verification of learning takes place through an oral interview, where the quality of the content, the property of the language and the ability to connect the topic with others treated in the program will be assessed. Verification of learning can also be carried out electronically, should the conditions require it

Not eligible - Knowledge and understanding of the topic: Important shortcomings. Significant inaccuracies. Ability to analyze and synthesize: Irrelevant. Frequent generalizations. Inability to synthesize,. Use of references: Completely inappropriate

 18-20 - Knowledge and understanding of the topic: At the threshold level. Obvious imperfections. Analysis and synthesis skills: Just enough skills. Use of references: As appropriate 

21-23 - Knowledge and understanding of the topic: Routine knowledge Analysis and synthesis skills: It is capable of correct analysis and synthesis. Argue logically and consistently- Using references: Use standard references

24-26 - Knowledge and understanding of the topic: Good knowledge. Analysis and synthesis skills: Has good analysis and synthesis skills. The arguments are expressed consistently. Using references: Use standard references

27-29 - Knowledge and understanding of the topic: Knowledge more than good. Ability to analyze and synthesize: He has considerable abilities of analysis and synthesis. Use of references: Has explored the topics. 

30-30L - Knowledge and understanding of the topic: Excellent knowledge. Ability to analyze and synthesize: He has considerable abilities of analysis and synthesis. Use of references: Important insights.

Examples of frequently asked questions and / or exercises

- Definition of "bioprocess"

- What are microbial biomass and an example of industrially produced microbial biomass

- The fermenter, operation and main components

- The main substrates used in an industrial bioprocess