BIOTECNOLOGIE MICROBICHE E AGROALIMENTARIModule GENETICA DEI MICRORGANISMI
Academic Year 2024/2025 - Teacher: Cinzia CAGGIAExpected Learning Outcomes
To conduct laboratory activities that use molecular methodologies in the agro-industrial field. At the end of the course the student will be able to interpret the results obtained from the experiments. The jujment autonomy and communication skills will be stimulated through the drafting and presentation of a laboratory report.
Course Structure
The teaching includes 21 hours of frontal lessons and 42 hours of exercises.
Teaching could be carried out in a mixed mode, also remotely, if required changes could be introduced with respect to previous statements, in line with the planned programme.
The planned activities concern practical exercises, laboratory tests, guided tours, in-depth seminars, group work. If the teaching is given in a mixed or remote way, the necessary changes with respect to what has been declared may be introduced, in order to comply with the planned program.
To guarantee equal opportunities and in compliance with the laws in force, interested students can ask for a personal interview in order to plan any compensatory and / or dispensatory measures, based on the didactic objectives and specific needs. For this purpose, it is also possible to contact the CInAP (Center for Active and Participatory Inclusion - Services for Disabilities and / or SLDs) of our Department, Prof. Anna De Angelis.
Required Prerequisites
Attendance of Lessons
Risultati della traduzione
Detailed Course Content
Microorganisms of biotechnological interest. Microorganisms as a "cell factory" for the production of goods and services. Research and development in industrial processes screening and optimization of production processes. Environmental issues and applications of microorganisms to protect the environment. Genetic improvement of microorganisms used in food industry. Development of microbial starters for the optimization of food fermentation. Basic concepts of bacterial genetics and effects of recent discoveries on the development of biotechnology.
Contribution of teaching to the goals of the 2030 Agenda for Sustainable Development. In particular, during the course, the issues related to the use of microorganisms as a strategy for achieving Goal 2: eradicate hunger in the world, achieve food security, improve nutrition and promote sustainable agriculture will be explored.
Textbook Information
Testo 1 Brock – Biologia dei Microrganismi –Microbiologia generale, ambientale e industriale - Ed Pearson
Testo 2 Glick BR, Pasternak JJ. - Biotecnologia Molecolare- Principi e applicazioni del DNA ricombinante- Ed Zanichelli
Testo 3 Donadio S, Marino G. Biotecnologie Microbiche – Ed CEA
Testo 4. Manzoni M, Microbiologia Industriale – Ed. CEA
Course Planning
Subjects | Text References | |
---|---|---|
1 | Bacterial genetics and biotechnologies. Microrganisms as cell factories. Microbial exploitation in biotech. Some cases. | Lesson's materials.Text n. 1 |
2 | The microbial world and taxonomic procedures.Prokaryotic and eukaryotic cells. Microbial diversity as biotechnological resource. Bacterial classification. | Lesson's materials. Text n. 2 |
3 | * Genetic variability. the mutations. Ames test. Fluctuations test. Replica plating. Mutazioni, mutageni e mutant | Lesson's materials. Text n. 1. |
4 | Griffith's experiment. Transformation. | L |
5 | La variablita' genetica. Fenomeni di trasposizione del DNA. Conjugation, transformation and transduction in bacteria. | Lesson's materials. Text n. 1. |
6 | Controllo dell'espressione genica. Gli operoni. L'operone lattosio e l'operone triptofano in Escherichia coli. | Lesson's materials. Text. n. 1 |
7 | Yeasts and their use in biotechnology. Saccharomyces cerevisiae. Homotallism and heterothallism. | Lesson's materials. Text. n. 1. |
8 | Laboratory experiences. Choice of a natural matrix. Count, isolation and identification of the main microbial species. Phenotypic and genotypic tests. |
Learning Assessment
Learning Assessment Procedures
The assessment of learning of the course takes place through oral examinations.
The student's preparation will be evaluated based on the following criteria: level of knowledge and deepening of the topics, properties of language and exposure, and reasoning skills developed by the student.
The final mark will be the average of the votes obtained in the single module, according to the following structure:
Unsuitable
Topic knowledge and understanding: Significant deficiencies.
Significant inaccuracies
Analysis and synthesis skills: Irrelevant. Frequent generalizations. Inability to synthesize
Using references: Completely inappropriate
18-20
Topic knowledge and understanding: Threshold level. Obvious imperfections
Analysis and synthesis skills: Barely sufficient capacity
Using references: Just appropriate
21-23
Topic knowledge and understanding: Routine knowledge
Analysis and synthesis skills: capacity of correct analysis and synthesis. Argues logically and coherently
Using references: Use standard references
24-26
Topic knowledge and understanding: Good knowledge
Analysis and synthesis skills: Has good analytical and synthesis skills. Topics are expressed coherently
Using references: Use standard references
27-29
Topic knowledge and understanding: Detectable neat knowledge
Analysis and synthesis skills: Has remarkable analytical and synthesis skills
Using references: Has delved into the topics
30-30L
Topic knowledge and understanding: Evident general knowledge
Analysis and synthesis skills: Has remarkable analytical and synthesis skills
Using references: Significant insights